Cancer

Cancer research

Live-cell imaging
in cancer research

  • Cancer Research

Cancer is characterized as a complex disease that results from a series of successive genetic mutations, leading to defective cell functions and proliferation. The development of novel scientific techniques, alongside the optimization of already existing approaches drives the advancement of cancer research.

Live-cell microscopy has become an indispensable tool for improving our understanding of cancer pathogenesis. The applications of time-lapse imaging range from directly visualizing the transient interactions between immune and cancer cells, to examining more complex biological processes, such as metastasis, programmed cell death and tumor angiogenesis.

Multi-well clonogenic assay to evaluate cytotoxicity and genotoxicity of anti-cancer agents

Label-free, semi-automated assessment of cell survival and colony formation

Clonogenic or colony formation assay is a widely used method in the field of cancer biology. It evaluates the effects of different treatments on the ability of cancer cells to survive and form colonies. Standard clonogenic protocols involve colony fixation, staining and examination at the end of a lengthy incubation period. The CytoSMART Omni allows continuous monitoring of clonogenic growth by creating time-lapse videos for days or even weeks at a time, without the need to fix or stain your cells. Monitoring the entire process of colony formation can provide a lot of valuable information, including the time-dependent effects of different drug treatments. In addition, the integrated image analysis software automatically detects newly formed colonies and evaluates their size and circularity.

Fluorescence live-cell microscopy in cancer immunotherapy

Labelled imaging of cell-cell interactions

Cyto SMART Lux3 FL fluorescence

Cancer immunotherapy harnesses the inherent capabilities of the host immune system to combat cancer. Fluorescence microscopy has been playing a key role in facilitating the understanding of interactions that take place between immune and tumor cells, as this cellular interplay represents the core of any immunotherapeutic approach. The CytoSMART Lux3 FL fluorescence imaging system can capture spatial interactions between different types of cells by gathering kinetic data in real time. As the imaging can take place directly from inside an incubator, cellular interactions are not affected by undesired extrinsic factors.

In vitro cell migration assay for studying cancer metastasis

Algorithm-based analysis of cell migration

Cancer metastasis involves the spread of malignant cells from their original site and is responsible for the largest number of cancer-related deaths. Numerous in vitro assays have been developed to replicate different stages of metastasis under a more controlled environment. Scratch assays (also known as a wound healing assays), in particular, can aid the analysis of cancer cell migration under the influence of various chemokines and growth factors. All CytoSMART live-cell imaging platforms (CytoSMART Omni, Lux2 or Lux3 FL) are equipped with the Scratch Assay software that can analyse the rate and efficiency of scratch closure and cell migration.

Mar4 2
3D cell cultures for cancer modelling

Tumor organoids represent a more sophisticated model for cancer research, as they more accurately mimic the genetic, cellular, and pathophysiological characteristics of cancer compared to traditional 2D cell cultures. CytoSMART Technologies has developed effective solutions for working with tumor-derived organoids. The CytoSMART Omni bright-field imaging system can closely monitor the process of tumor organoid formation by recording whole-vessel time-lapse videos directly from inside an incubator. The Corning Cell Counter, adapted with the innovative Organoid Counting software, can determine the precise number and size of organoids in a sample in no time.

Videos

Appnotes

Cell Cytoxicity Assay to Analyze Drug Response

Evaluating Drug Toxicity Using Label-Free Microscopy
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Cell Migration

Live-Cell Imaging and Quantitative Analysis of Wound Healing Assays
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Cell Viability Analysis

Comparison Between Metabolic Activity Assay and Confluence Analysis
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