Live-cell imaging for high-throughput experiments
Many neurodegenerative diseases are associated with the loss of neurons. Understanding the sequence of events that lead to cell death is imperative for disease research. By analyzing the health of neurons over time under different conditions neuroscientists can identify neuroprotective and/or neurotoxic events. The CytoSMART Omni, which can scan full flask surfaces, uses time-lapse imaging to continuously monitor neurons for up to weeks at a time. This system negates the need to fix or stain cells meaning it can be used to study the time-dependent effects of different therapeutic treatments in real time and from within the culture environment.
Brain organoids — 3D cell cultures in neuroscience research
3D cell cultures of brain organoids, which are derived from embryonic stem cells and pluripotent stem cells, mimic the brain more accurately than traditional 2D cell cultures. Brain organoids are used by neuroscientists to investigate brain development and function, disease pathogenesis and gene expression. The CytoSMART Lux3 FL fluorescence imaging microscope has been adapted to automatically generate time-lapse movies from inside the incubator. This system can be used to track the expression of fluorescent proteins within cultures without disturbing the organoids.
Fluorescence live-cell microscopy in neuroscience research
By studying how the different cells within the brain interact, neuroscientists can gain insight into many diseases, including cancer, Alzheimer’s disease, and multiple sclerosis. The complex morphology of brain cells makes studying cell-cell interactions difficult. Fluorescence microscopy has had a pivotal role in defining the interactions between neurons and other brain cells. Live-cell imaging systems, such as the CytoSMART Lux3 FL fluorescence imaging system, can gather kinetic data in real time from inside an incubator. This system enables neuroscientists to track cell-cell interactions without needing to disrupt the cells or remove them from their culture environment.