Organoids are three-dimensional (3D) cell structures that contain a multitude of organ-specific cells, that are formed by self-organisation and differentiation of stem cells. Organoids are better at representing cellular environments found in vivo than conventional two-dimensional (2D) cell cultures.……. Read more
Cell counting is a necessary step for in vitro work involving cell culture. Using the correct organoid concentration will ensure that downstream experiments are reproducible and accurate. Organoid counts are important for monitoring overall health and proliferation rates, seeding for experiments and preparing for assays. Organoids can be counted using a glass slide or hemocytometer and cover slip. In some instances organoids can be too large for the hemocytometer and can be counted in droplets on a glass slide.
While it is a necessary part of organoid culture, counting can be tedious and is time-consuming. For researchers looking for an automated solution, we recommend the Corning Cell Counter equipped with organoid counting software. Use a hemocytometer or a simple glass slide to count organoids using an image based analysis.
Initial characterization can be made using a light microscope. Using a straightforward time-lapse imaging solution researchers can monitor basic morphological features of whole wells in multi-well plates. The CytoSMART Omni is designed for just this purpose and operated from within cell culture incubators.
Corning Cell Counter Automated Organoid Counting Appnote
Manually counting organoids is a routine part of laboratory operations, but is time consuming and user dependent. To overcome these issues CytoSMART has introduced an automated organoid counting applications that works with common reusable glass hemocytometers.
Here we discuss the features of the organoid counting application and it's functions on the Corning Cell Counter.