The CytoSMART Lux3 FL is a small fluorescence live-cell imaging
microscope equipped with one brightfield and two fluorescent channels
(green and red). The device enables researchers to unravel cellular
processes in real-time, providing lots of valuable data about the
experiments and answers if, when, how, and to what extend the cellular
processes occur. CytoSMART’s first fluorescence live-cell imager allows
users to track dynamic cellular processes with high specificity by
taking high-quality images to create real-time time-lapse movies.
Simultaneously, the cells are kept in a controlled environment inside a
standard cell culture incubator.
Let Lux3 FL capture fluorescent and brightfield images for you
Even when you are not in the lab
Currently, fluorescent labeling is mostly used as an end-point measurement. However, time-lapse imaging of live cells can give much more information about biological processes. By using automated imaging at regular time intervals, the temporal resolution of the fluorescent data is increased, leading to even more relevant data about the cellular processes. In this way, researchers can not only determine if a certain process has occurred, but also when it occurred and at what speed.
Applications of kinetic fluorescence assays
The use of fluorescence live-cell imaging has become increasingly
valuable in the fields of drug discovery, tissue engineering,
immunology, immunotherapy, and cancer research. Various applications,
such as monitoring cell viability, determining transfection efficiency,
investigating co-cultures, and analyzing cellular processes, can benefit
from the fluorescent labels and their detailed kinetic visualization.
Many of these applications can also benefit from the integrated image
analysis included in the CytoSMART Cloud. Imagine effortless
calculations of transfection efficiency or the percentage of apoptotic
More examples of applications can be found in the guide below.
Analyze cells in their desired culture environment
Combine fuorescence and brighflield live-cell imaging inside the same incubator
Kinetic fluorescent imaging of cellular processes requires environmental control throughout the experiment. Because of its small size, the Lux3 FL fits inside any standard incubator. This enables you to perform fluorescent live-cell imaging experiments parallel to your regular cell cultures. Because of the remote data accessibility in the CytoSMART Cloud, your cells do not have to be disturbed during the experiment. This makes sure the cells are in the most optimal culture conditions as possible. Next to this, the remote data accessibility prevents you from making unnecessary lab visits.
Simultaneous analysis of multiple cell features
Expand the number of variables you can analyze in your cell culture
Using CytoSMART Lux3 FL, you can analyze green and red fluorescent tags simultaneously with your brightfield images. This not only saves you time but also provides tools to distinguish cells from each other or distinguish labeled structures within the cells. By tracking changes in the fluorescent signal over time, cell number changes or fluorescently tagged cellular components can easily be identified.
Lux3 FL brochure 148x148mm spread
Lux3-FL-brochure-148x148mm-sprea ( PDF - 5 MB )
Appnote 0001 Studying the effects of H₂ O₂ on the apoptotic pathway of CHO K1 cells using fluorescence live cell imaging
Appnote-0001-Studying-the-effects-of-H₂O₂-on-the-apoptotic-pathway-of-CHO-K1-cells-using-fluorescence-live-cell-imaging ( PDF - 2 MB )
Appnote 0002 The effect of fibroblast co culture on Hep G2 growth
Appnote-0002-The-effect-of-fibroblast-co-culture-on-HepG2-growth ( PDF - 3 MB )
CASE STUDY Fluorescence imaging 3d cell culture for drug screening
Case-study-Fluorescence-imaging-3d-cell-culture-for-drug-screening_2020-12-03-093515 ( PDF - 1 MB )
Cyto SMART Lux3 FL application guide PDF
CytoSMART-Lux3-FL-application-guide-PDF ( PDF - 8 MB )
Lux3 FL 1 pager example application
lux3-FL-1-pager-example-application ( PDF - 738 KB )
Frequently Asked Questions
The CytoSMART Lux3 FL is a small fluorescence live-cell imaging microscope equipped with one brightfield and two fluorescent channels (green and red). The device allows users to label and track dynamic cellular processes with high specificity by taking high-quality images to create real-time time-lapse movies. Experiments' data can be accessed and analyzed remotely via the CytoSMART Cloud outside the lab.
The Lux3 FL can image and analyze various applications, for example, cell viability, transfection, co-culture, cellular processes, nanoparticles. Researchers working in drug discovery, tissue engineering, immunology, immunotherapy, and cancer research fields can benefit from the fluorescence live-cell imaging.
Yes, viability is one of the numerous applications that fluorescence live-cell imaging has to offer.
If fluorescent die affects cell viability depends on the dye that is used. In general, when a live cell imaging dye is developed, cell viability is always taken into account. Of course, you don’t want the dye to affect the cells negatively. There are various fluorescent live-cell imaging dyes on the market, and we can share our recommendations. Feel free to contact us for more information.
Many different fluorescent dyes can be used, as long as the fluorescent dye's excitation and emission spectrum correspond with the wavelength of the LED and detector of the Lux3 FL. Some examples are RFP (red fluorescent protein), PI, Cell Tracker Orange, Cell Tracker Red for the red channel, and GFP (green fluorescent protein), Cell Tracker green for the green channel.
It is essential to match the fluorescent dye with the specifications of the device. E.g. we don't recommend using Hoechst or DAPI since they are blue dyes. Next to this, it is important to ensure that the dye is not toxic for the cells since you want to use it on living cells.
Magnification is equal to a standard microscope with a 10X and 20X lens.
Yes, with the Lux3 FL, it's possible to set the LEDs intensity for all channels (brightfield, red fluorescent channel, and green fluorescent) according to users' preferences.
Both fluorescence channels are included in the price. Researchers can make a time-lapse (or a single snap) with the brightfield, red and green channel imaging in parallel. Of course, it is also possible to choose just one of the fluorescent channels, depending on the experiment's goal and the researcher's preferences.
Images can be recorded at pre-defined intervals. At the start of a new experiment, you can specify the interval rate anywhere between 5 min - 12 hours.
The CytoSMART Lux3-fl allows monitoring of a wide range of different culture dishes and flasks, such as:
- T-flasks: T-25 up to T-250
- Single well, multi-well plates (6, 12, 24, 96 well)
- microfluidic chips
- Flat tubes
- Petri dishes
For larger flasks an adapter plate may be required. This plate can be purchased at CytoSMART.
The system requires a dedicated PC or laptop running on windows 10 or above and with a USB3 port and an active internet connection. If your lab doesn’t have the sufficient hardware, we can supply these for a fee as part of your order.
The CytoSMART Lux3 FL remote functionality runs on cloud-based software. In this cloud-environment, the images and videos are stored and can be accessed using user-specific login details. Next to unlimited data storage, automated image analysis algorithms can be run to quantify confluence and cell migration.
The devices are easy to clean using lint-free wipes and EtOH (70%) or IPA. Do not use Acetone to clean the device. Please be aware that the device cannot be autoclaved. After sterilizing with EtOH or IPA the device can be used in a cleanroom.
This is not necessary. However, to get the best results, we advise covering the fluorescence microscope slightly so that the incidence of light from the side is attenuated as much as possible. If you cover the light arc using an open book, this will already suffice to visualize this.