This new application automatically detects organoids using bright-field image analysis
Together with Corning®, CytoSMART already introduced an automated cell counter for counting of mammalian cells called the Corning® Cell Counter.
A new, powerful and simple software application has been developed that enables organoid counting with the Corning® Cell Counter.
Manually counting organoids is a routine part of laboratory operations, but is time consuming and user dependent. To overcome these issues CytoSMART has introduced an organoid counting application that is:
Fast & Accurate - thanks to its cloud-based image analysis algorithm
Low-cost - works with common reusable glass hemocytometer. No consumables required
Precise - software allows for data gating and image selection for statistical analysis
The Organoid Counting software can analyze a single image in less than three seconds* utilizing the CytoSMART™ Cloud. This cloud computing ability enables the rapid images analysis.
*at a 73 Mbps download and 20 Mbps upload speed
Organoid size can be highly variable, therefore it is advisable to take multiple images at various positions within the counting chamber.
To give an example: Suppose a single large organoid covers the Field Of View of a certain area while it is surrounded by smaller organoids. If a single image of that large organoid is used for the concentration estimation, the smaller organoids are neglected and organoid concentration is underestimated. Users can overcome that issue by quickly taking images at various locations in the counting chamber. Using the data obtained from multiple images, the accuracy of the organoid count drastically improves (fig 4).
Accessible Data Anywhere, Anytime
The Organoid Counting software instantly generates a report containing organoid number and size. The data is sent to the CytoSMART™ Cloud, which enables you to access the analyzed image data on your smartphone, tablet, or computer. Since all data is saved in the CytoSMART™ Cloud, you can gain insight in the population size and concentration from one experiment to the next.