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Collaboration: PromoCell and CytoSMART Technologies

Besides the CytoSMART Lux2 for monitoring general cell health and proliferation during culture, the CytoSMART Omni can be used to analyze experiments using primary cells. One example of this is the analysis of the migratory capacities of primary human keratinocytes. We cultured monolayers of PromoCell’s juvenile Normal Human Epidermal Keratinocytes in a 48-well plate and made a wound in the monolayer to mimic the re-epithelialization process, one of the first processes in cutaneous wound healing. Thereafter, wound closure was analyzed every hour using the CytoSMART Omni and its scratch analysis algorithm. An example of the keratinocyte wound closure can be found in this video:

If you are interested in the entire experiment and the results, please check out the application note we made together with PromoCell here:

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